BLASTN 2.4.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: rna.fa 176,426 sequences; 587,117,901 total letters Query= TTGAAGGTCGCAGAGTTCCT (evalue = 10) Length=20 Score E Sequences producing significant alignments: (Bits) Value gi|1034625752|ref|XM_017028038.1| PREDICTED: Homo sapiens tumor... 40.1 0.002 gi|1034625754|ref|XM_011529035.2| PREDICTED: Homo sapiens tumor... 40.1 0.002 gi|1034625751|ref|XM_011529034.2| PREDICTED: Homo sapiens tumor... 40.1 0.002 gi|345197263|ref|NM_001243892.1| Homo sapiens tumor protein D52... 40.1 0.002 gi|345197261|ref|NM_001243891.1| Homo sapiens tumor protein D52... 40.1 0.002 gi|345197259|ref|NM_199359.2| Homo sapiens tumor protein D52 li... 40.1 0.002 gi|345197256|ref|NM_199363.2| Homo sapiens tumor protein D52 li... 40.1 0.002 gi|345197254|ref|NM_199361.2| Homo sapiens tumor protein D52 li... 40.1 0.002 gi|345199272|ref|NR_045090.1| Homo sapiens tumor protein D52 li... 40.1 0.002 gi|345197255|ref|NM_003288.3| Homo sapiens tumor protein D52 li... 40.1 0.002 gi|345197253|ref|NM_199362.2| Homo sapiens tumor protein D52 li... 40.1 0.002 gi|345128623|ref|NM_199360.2| Homo sapiens tumor protein D52 li... 40.1 0.002 gi|345197267|ref|NM_001243894.1| Homo sapiens tumor protein D52... 36.2 0.030 gi|1034628108|ref|XM_017028533.1| PREDICTED: Homo sapiens growt... 28.2 7.3 gi|53988386|ref|NM_052869.1| Homo sapiens tweety family member ... 28.2 7.3 gi|1034602103|ref|XM_005257824.3| PREDICTED: Homo sapiens tweet... 28.2 7.3 gi|53988384|ref|NM_032646.5| Homo sapiens tweety family member ... 28.2 7.3 gi|1034643166|ref|XM_017008908.1| PREDICTED: Homo sapiens famil... 28.2 7.3 gi|193788652|ref|NM_001129891.1| Homo sapiens family with seque... 28.2 7.3 gi|1034643165|ref|XM_011534415.2| PREDICTED: Homo sapiens famil... 28.2 7.3 gi|1034554640|ref|XM_006711111.3| PREDICTED: Homo sapiens adeno... 28.2 7.3 gi|1034554641|ref|XM_006711113.2| PREDICTED: Homo sapiens adeno... 28.2 7.3 gi|1034554642|ref|XM_006711112.2| PREDICTED: Homo sapiens adeno... 28.2 7.3 gi|1034554643|ref|XM_017000037.1| PREDICTED: Homo sapiens adeno... 28.2 7.3 gi|767908089|ref|XM_011509062.1| PREDICTED: Homo sapiens adenos... 28.2 7.3 gi|1034554637|ref|XM_011509061.2| PREDICTED: Homo sapiens adeno... 28.2 7.3 gi|767908085|ref|XM_011509060.1| PREDICTED: Homo sapiens adenos... 28.2 7.3 gi|1034554639|ref|XM_006711109.2| PREDICTED: Homo sapiens adeno... 28.2 7.3 gi|301601657|ref|NM_001193495.1| Homo sapiens adenosine deamina... 28.2 7.3 gi|301601652|ref|NM_001111.4| Homo sapiens adenosine deaminase,... 28.2 7.3 gi|301601655|ref|NM_015840.3| Homo sapiens adenosine deaminase,... 28.2 7.3 gi|301601656|ref|NM_015841.3| Homo sapiens adenosine deaminase,... 28.2 7.3 gi|301601659|ref|NM_001025107.2| Homo sapiens adenosine deamina... 28.2 7.3 gi|1034673954|ref|XM_005274485.2| PREDICTED: Homo sapiens matri... 28.2 7.3 gi|201860266|ref|NM_015419.3| Homo sapiens matrix-remodelling a... 28.2 7.3 gi|1034632055|ref|XM_006713062.3| PREDICTED: Homo sapiens RAD54... 28.2 7.3 gi|1016080588|ref|NM_001322257.1| Homo sapiens RAD54-like 2 (S.... 28.2 7.3 gi|1034632051|ref|XM_017005987.1| PREDICTED: Homo sapiens RAD54... 28.2 7.3 gi|1034632053|ref|XM_017005988.1| PREDICTED: Homo sapiens RAD54... 28.2 7.3 gi|1016080528|ref|NM_001322256.1| Homo sapiens RAD54-like 2 (S.... 28.2 7.3 gi|1016470414|ref|NM_015106.3| Homo sapiens RAD54-like 2 (S. ce... 28.2 7.3 gi|1016080520|ref|NM_001322253.1| Homo sapiens RAD54-like 2 (S.... 28.2 7.3 > gi|1034625752|ref|XM_017028038.1| PREDICTED: Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant X2, mRNA Length=1110 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 626 TTGAAGGTCGCAGAGTTCCT 607 > gi|1034625754|ref|XM_011529035.2| PREDICTED: Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant X3, mRNA Length=1108 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 632 TTGAAGGTCGCAGAGTTCCT 613 > gi|1034625751|ref|XM_011529034.2| PREDICTED: Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant X1, mRNA Length=1181 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 701 TTGAAGGTCGCAGAGTTCCT 682 > gi|345197263|ref|NM_001243892.1| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 8, mRNA Length=2230 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 503 TTGAAGGTCGCAGAGTTCCT 484 > gi|345197261|ref|NM_001243891.1| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 7, mRNA Length=2284 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 557 TTGAAGGTCGCAGAGTTCCT 538 > gi|345197259|ref|NM_199359.2| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 6, mRNA Length=2299 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 572 TTGAAGGTCGCAGAGTTCCT 553 > gi|345197256|ref|NM_199363.2| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 4, mRNA Length=2341 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 614 TTGAAGGTCGCAGAGTTCCT 595 > gi|345197254|ref|NM_199361.2| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 2, mRNA Length=2368 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 641 TTGAAGGTCGCAGAGTTCCT 622 > gi|345199272|ref|NR_045090.1| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 11, non-coding RNA Length=2328 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 601 TTGAAGGTCGCAGAGTTCCT 582 > gi|345197255|ref|NM_003288.3| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 5, mRNA Length=2359 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 632 TTGAAGGTCGCAGAGTTCCT 613 > gi|345197253|ref|NM_199362.2| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 3, mRNA Length=2401 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 674 TTGAAGGTCGCAGAGTTCCT 655 > gi|345128623|ref|NM_199360.2| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 1, mRNA Length=2428 Score = 40.1 bits (20), Expect = 0.002 Identities = 20/20 (100%), Gaps = 0/20 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTCCT 20 |||||||||||||||||||| Sbjct 701 TTGAAGGTCGCAGAGTTCCT 682 > gi|345197267|ref|NM_001243894.1| Homo sapiens tumor protein D52 like 2 (TPD52L2), transcript variant 9, mRNA Length=2197 Score = 36.2 bits (18), Expect = 0.030 Identities = 18/18 (100%), Gaps = 0/18 (0%) Strand=Plus/Minus Query 1 TTGAAGGTCGCAGAGTTC 18 |||||||||||||||||| Sbjct 470 TTGAAGGTCGCAGAGTTC 453 > gi|1034628108|ref|XM_017028533.1| PREDICTED: Homo sapiens growth arrest specific 2 like 1 (GAS2L1), transcript variant X1, mRNA Length=3192 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 226 TGAAGGTCGCAGAG 213 > gi|53988386|ref|NM_052869.1| Homo sapiens tweety family member 2 (TTYH2), transcript variant 2, mRNA Length=3278 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 7 GTCGCAGAGTTCCT 20 |||||||||||||| Sbjct 3088 GTCGCAGAGTTCCT 3075 > gi|1034602103|ref|XM_005257824.3| PREDICTED: Homo sapiens tweety family member 2 (TTYH2), transcript variant X1, mRNA Length=3375 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 7 GTCGCAGAGTTCCT 20 |||||||||||||| Sbjct 3206 GTCGCAGAGTTCCT 3193 > gi|53988384|ref|NM_032646.5| Homo sapiens tweety family member 2 (TTYH2), transcript variant 1, mRNA Length=3468 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 7 GTCGCAGAGTTCCT 20 |||||||||||||| Sbjct 3278 GTCGCAGAGTTCCT 3265 > gi|1034643166|ref|XM_017008908.1| PREDICTED: Homo sapiens family with sequence similarity 196 member B (FAM196B), transcript variant X2, mRNA Length=2726 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 5 AGGTCGCAGAGTTC 18 |||||||||||||| Sbjct 2385 AGGTCGCAGAGTTC 2372 > gi|193788652|ref|NM_001129891.1| Homo sapiens family with sequence similarity 196 member B (FAM196B), mRNA Length=3548 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 5 AGGTCGCAGAGTTC 18 |||||||||||||| Sbjct 2617 AGGTCGCAGAGTTC 2604 > gi|1034643165|ref|XM_011534415.2| PREDICTED: Homo sapiens family with sequence similarity 196 member B (FAM196B), transcript variant X1, mRNA Length=5648 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 5 AGGTCGCAGAGTTC 18 |||||||||||||| Sbjct 2306 AGGTCGCAGAGTTC 2293 > gi|1034554640|ref|XM_006711111.3| PREDICTED: Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant X5, mRNA Length=6567 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5496 TGAAGGTCGCAGAG 5483 > gi|1034554641|ref|XM_006711113.2| PREDICTED: Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant X6, mRNA Length=6343 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5272 TGAAGGTCGCAGAG 5259 > gi|1034554642|ref|XM_006711112.2| PREDICTED: Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant X7, mRNA Length=6269 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5198 TGAAGGTCGCAGAG 5185 > gi|1034554643|ref|XM_017000037.1| PREDICTED: Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant X8, mRNA Length=6387 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5287 TGAAGGTCGCAGAG 5274 > gi|767908089|ref|XM_011509062.1| PREDICTED: Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant X4, mRNA Length=6497 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5397 TGAAGGTCGCAGAG 5384 > gi|1034554637|ref|XM_011509061.2| PREDICTED: Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant X2, mRNA Length=6459 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5359 TGAAGGTCGCAGAG 5346 > gi|767908085|ref|XM_011509060.1| PREDICTED: Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant X1, mRNA Length=6570 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5470 TGAAGGTCGCAGAG 5457 > gi|1034554639|ref|XM_006711109.2| PREDICTED: Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant X3, mRNA Length=6474 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5374 TGAAGGTCGCAGAG 5361 > gi|301601657|ref|NM_001193495.1| Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant 5, mRNA Length=6730 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5606 TGAAGGTCGCAGAG 5593 > gi|301601652|ref|NM_001111.4| Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant 1, mRNA Length=6692 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5568 TGAAGGTCGCAGAG 5555 > gi|301601655|ref|NM_015840.3| Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant 2, mRNA Length=6614 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5490 TGAAGGTCGCAGAG 5477 > gi|301601656|ref|NM_015841.3| Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant 3, mRNA Length=6557 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5433 TGAAGGTCGCAGAG 5420 > gi|301601659|ref|NM_001025107.2| Homo sapiens adenosine deaminase, RNA-specific (ADAR), transcript variant 4, mRNA Length=6561 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Minus Query 2 TGAAGGTCGCAGAG 15 |||||||||||||| Sbjct 5437 TGAAGGTCGCAGAG 5424 > gi|1034673954|ref|XM_005274485.2| PREDICTED: Homo sapiens matrix remodeling associated 5 (MXRA5), transcript variant X1, mRNA Length=10243 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 3 GAAGGTCGCAGAGT 16 |||||||||||||| Sbjct 2890 GAAGGTCGCAGAGT 2903 > gi|201860266|ref|NM_015419.3| Homo sapiens matrix-remodelling associated 5 (MXRA5), mRNA Length=9800 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 3 GAAGGTCGCAGAGT 16 |||||||||||||| Sbjct 2435 GAAGGTCGCAGAGT 2448 > gi|1034632055|ref|XM_006713062.3| PREDICTED: Homo sapiens RAD54-like 2 (S. cerevisiae) (RAD54L2), transcript variant X3, mRNA Length=9740 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 4 AAGGTCGCAGAGTT 17 |||||||||||||| Sbjct 5450 AAGGTCGCAGAGTT 5463 > gi|1016080588|ref|NM_001322257.1| Homo sapiens RAD54-like 2 (S. cerevisiae) (RAD54L2), transcript variant 4, mRNA Length=9849 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 4 AAGGTCGCAGAGTT 17 |||||||||||||| Sbjct 5543 AAGGTCGCAGAGTT 5556 > gi|1034632051|ref|XM_017005987.1| PREDICTED: Homo sapiens RAD54-like 2 (S. cerevisiae) (RAD54L2), transcript variant X1, mRNA Length=9769 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 4 AAGGTCGCAGAGTT 17 |||||||||||||| Sbjct 5479 AAGGTCGCAGAGTT 5492 > gi|1034632053|ref|XM_017005988.1| PREDICTED: Homo sapiens RAD54-like 2 (S. cerevisiae) (RAD54L2), transcript variant X2, mRNA Length=11329 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 4 AAGGTCGCAGAGTT 17 |||||||||||||| Sbjct 7039 AAGGTCGCAGAGTT 7052 > gi|1016080528|ref|NM_001322256.1| Homo sapiens RAD54-like 2 (S. cerevisiae) (RAD54L2), transcript variant 3, mRNA Length=9947 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 4 AAGGTCGCAGAGTT 17 |||||||||||||| Sbjct 5641 AAGGTCGCAGAGTT 5654 > gi|1016470414|ref|NM_015106.3| Homo sapiens RAD54-like 2 (S. cerevisiae) (RAD54L2), transcript variant 2, mRNA Length=10009 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 4 AAGGTCGCAGAGTT 17 |||||||||||||| Sbjct 5703 AAGGTCGCAGAGTT 5716 > gi|1016080520|ref|NM_001322253.1| Homo sapiens RAD54-like 2 (S. cerevisiae) (RAD54L2), transcript variant 1, mRNA Length=10057 Score = 28.2 bits (14), Expect = 7.3 Identities = 14/14 (100%), Gaps = 0/14 (0%) Strand=Plus/Plus Query 4 AAGGTCGCAGAGTT 17 |||||||||||||| Sbjct 5751 AAGGTCGCAGAGTT 5764 Lambda K H 1.37 0.711 1.31 Gapped Lambda K H 1.37 0.711 1.31 Effective search space used: 2337180340 Database: rna.fa Posted date: Jan 6, 2017 3:58 PM Number of letters in database: 587,117,901 Number of sequences in database: 176,426 Matrix: blastn matrix 1 -3 Gap Penalties: Existence: 5, Extension: 2